EKSPLORASI BAKTERI PROTEOLITIK DARI ILEUM GALLUS GALLUS SEBAGAI KANDIDAT AGEN PROBIOTIK PAKAN TERNAK UNGGAS LOKAL
Abstract
This study aims to exploration and investigate proteolytic bacteria from ileum of Gallus gallus as probiotic agent candidates in local poultry feed. This research was conducted in the Village of Klambir V and Laboratory at Pembangunan Panca Budi University, Medan. The exploratory research method included bacterial isolation, activity of proteolytic bacterial and proteolytic bacterial staining. The results showed that there were twenty proteolytic isolates with different morphological characteristics of the bacteria. Ten isolates belong to the group of gram-positive bacteria and ten isolates belong to the group of gram-negative bacteria. There are three isolates with the highest enzyme production, there are GP sp. 1, GP sp. 3 and GP sp. 4 with Proteolytic Index (IP) value of 1,500, 1,533, and 1,531 and the three isolates with the lowest enzyme production in GP sp. 9, GP sp. 11 and GP sp. 13 with Proteolytic Index (IP) values of 0.696, 0.641, and 0.562. Based on the research results, GP sp. 1, GP sp. 3 and GP sp. 4 has high enzyme production and include to the group of gram-positive bacteria so that, three isolates have the potential to be candidates for probiotic agents for local poultry feed.
References
Akhdiya, A. 2003. Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil. Buletin Plasma Nutfah, 9 (2): 38-44.
Djide, M. N dan Sartini, 2005, Mikrobiologi Farmasi Dasar, Laboratorium Mikrobiologi Farmasi Universitas Hasanuddin, Makassar.
Dwidjoseputro, D. 2011. Dasar-Dasar Mikrobiologi. Surabaya: Djambatan.
Eckel, E, F dan Ametaj, B, N. 2020. Bacterial Endotoxins and Their Role in Periparturient Diseases of Dairy Cows: Mucosal Vaccine Perspectives. Dairy 1(1): 61–90.
Fitriana, N dan Asri, M. 2022. Aktivitas Proteolitik pada Enzim Protease dari Bakteri Rhizosphere Tanaman Kedelai (Glycine max L.) di Trenggalek. LenteraBio, 11(1): 144-152.
Gill, Sher Singh., Shrivastav, Archana., dan Jana, Asha Mukul. 2016. Isolation and Identification of Protease producing Bacteria through Biodegradation of Protein content of Kitchen Wastes in Gwalior, India. International Journal of Current Microbiology and Applied Sciences, 5 (10): 204-211.
Hameed, A.A. Abdel, A.A. Shakour dan H.I. Yasser. 2003. Evaluation of bio- aerosols at an animal feed manufacturing industry: A case study. Aerobiologia, 1(19): 89–95.
Hastuti, U. S., Sarwendah., Febriani., Nugraheni., Asri., Asna dan Putri, M. 2017. Identifikasi dan Penentuan Indeks Hidrolisis Protein pada Bakteri Proteolitik dari Tanah Mangrove di Margomulyo, Balikpapan. Proceeding Biology Education Conference, 14 (1): 265 – 270.
Mani V., Weber, T. E., Baumgard, L. H., dan Gabler, N. K. 2012. Growth and Development Symposium: Endotoxin, inflammation, and intestinal function in livestock. J ANIM SCI, 90(1):1452-1465.
Nurmalinda A., Periadnadi, dan Nurmiati. 2013. Isolasi dan Karakterisasi Parsial Bakteri Indigenous Pemfermentasi dari Buah Durian (Durio zibethinus Murr.). Jurnal Biologi Universitas Andalas, 2(1): 8-13.
Rao, M.B., Tangsale, A.M., Ghasge, M.S., Deshpande, V.V. 1998. Molecular and Biotechnology Aspects of Microbial Protease. Microb Mol Biol Rev 62(1): 597–635.
Rizaldi. Rachmat, Woro Hastuti Setyantini, dan Sudarno. 2018. Isolasi dan Karakterisasi Bakteri Proteolitik yang Berasosiasi dengan Lamun (Enhalus acoroides) di Pantai Bama, Taman Nasional Baluran, Situbondo, Jawa Timur. JIPK, 10 (1): 1-10.
Semaun, R. 2021. Kajian Bahan Bioaktivator Limbah Buah Mengkudu Dalam Pupuk Organik Cair Terhadap Produksi Rumput Gajah Mini (Pennisetum Purpureum Cv. Mott) Sebagai Pakan Kambing. Disertasi. Program Doktor Ilmu Pertanian Sekolah Pascasarjana Universitas Hasanuddin Makassar.
Sony, I. S. dan V. P. Potty. 2016. Isolation and Identification of Protease Producing Bacteria from Food Processing Industries. International Journal of Current Microbiology and Applied Sciences, 5(3):181-189.
Suhartono. 1991. Protease. Bogor: IPB Press.
Susanti, E. V. H. 2003. Isolasi dan Karakterisasi Protease dari Bacillus subtilis 1012M15. Jurnal Biodiversitas, 4(1): 12-17.
Susatyo, I., D. 2006. Isolasi dan Identifikasi Bakteri Gelatinolitik Asal Tambak Daerah Gresik dan lamongan. Surabaya: Universitas Airlangga.
Susilo, H. 2012. Fisiologi Tanaman Budidaya. Jakarta: Universitas Indonesia Press.
Sutedjo, M. 1996. Mikrobiologi Tanah. Jakarta: Rineka Cipta.
Sutoro, Y., Soelaeman dan Iskandar. 2010. Budidaya Tanaman Jagung. Bogor: Balitbang Tanaman Pangan.
Suyasa, I. W. B. 2011. Isolasi bakteri pendegradasi minyak/lemak dari beberapa sedimen perairan tercemar dan bak penampungan limbah.
Yusmarini, Retno Indrati, Tyas Utami, dan Yustinus Marsono. 2009. Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan. Jurnal Natur Indonesia, 12(1): 28-33.
